NEW! Technical Article No. 12
Simultaneous LC/MS Analyses of Phosphorylated Saccharides
Phosphorylated saccharides take the important role as intermediates in various metabolic pathways of animals. For example, during glycolysis process, glucose is first converted to glucose-6-phosphate, then to other phosphorylated saccharides (such as fructose-6-phosphate and fructose-1,6-bisphosphate) and finally to pyruvate to produce ATP, the energy source of cell processing. Through glycogenolysis process, glucose-1-phosphate is produced from breakdown process of glycogen branches. In this process, glucose-6-phosphate is also produced as an isomer, and the product goes through a glycolysis process via different pathway. High sensitive analysis of phosphorylated saccharides in vivo is important for understanding the biological phenomena and early detection and treatment of the related diseases. One such case is glycogenosis, which stores abnormal amounts of glycogen in the body, and is possibly related to genetic glycogen enzymes deficiencies. Newborn glucose-6- phosphate concentration monitoring is considered an effective tool for the early detection of the disease.
In this application, we used a combination of Shodex™ HILICpak™ columns and LC/MS for simultaneous and high sensitive analysis of various phosphorylated saccharides without the use of ion-pair reagent nor derivatization.
✓ Phosphorylated Saccharides Using HILICpak™ VT-50 2D (glucose-1-phosphate (G1P), glucose-6-phosphate (G6P), fructose-1-phosphate (F1P), fructose-6-phosphate (F6P), fructose-1,6-bisphosphate (F1,6bP), and uridine diphosphate glucose (UDP-Glc));
✓ Phosphorylated Saccharides Using HILICpak™ VG-50 2D (fructose, glucose, G1P, G6P, UDP-Glc, and F1,6bP)
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