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NEW! Technical Article No. 6
Simultaneous Analysis of Ten Water-Soluble Vitamins Using a Polymer-Based Reversed Phase Column Shodex™ RSpak DE-413L
Methods using microbiological assays, absorption spectrophotometry, and HPLC are laborsome and time consuming process. Use of ODS column with an addition of ion-pair reagent tends to remain on the column and the flow-lines, results in increased background level and lowers the sensitivity. In this application, we aimed to develop a simple method to simultaneously analyze various water-soluble vitamins. We used Shodex™ RSpak DE-413L, a polymer-based reversed phase column, without a use of ion-pair reagent.
Click here to read the full PDF version >>> Chromato News FEB 2019 – English