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SHODEX Chromato News September 2022

news | 14 September 2022
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Effects of Flow Rate on Separation (GPC HK-401: PS) >> Application Note

☆ Effects of Flow Rate on Separation (GPC HK-404L: PS) >> Application Note

★ Effects of Flow Rate on Separation (GPC HK-406: PS) >> Application Note

☆ Analysis of Ibandronate Sodium (IC I-524A) >> Application Note

Amino Acids (3) (CXpak P-421S) >> Application Note

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SHODEX Chromato News August 2022

news | 22 August 2022
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Effects of Flow Rate on the Column Pressure (GPC HK-400 series) >> Application Note

☆ Effects of Column Unit on Separation (GPC HK-401: PS) >> Application Note

★ Effects of Column Unit on Separation (GPC HK-404L: PS) >> Application Note

☆ Effects of Column Unit on Separation (GPC HK-406: PS) >> Application Note

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SHODEX – Thailand LAB INTERNATIONAL (2022)

event | 16 August 2022
Title:

Thailand LAB INTERNATIONAL

Venue:

Bangkok International Trade & Exhibition Centre (BITEC) – Bangkok, Thailand

Date:

14 – 16 September 2022 | 10.00 am – 05.00 pm

Booth No.:

3H15

Tag: food and beverage Pharmaceutical Shodex Thailand

Shodex Singapore (Booth No. 3H15) will join Thailand LAB INTERNATIONAL held at Bangkok International Trade & Exhibition Centre (BITEC) from 14 to 16 September 2022.

Thailand LAB INTERNATIONAL is one of the leading laboratory exhibitions in Asia, showcasing all laboratory equipment and instrument covering areas of Analytical & Testing, Calibration & Metrology, Clinical & Diagnostic, Environmental & Safety, and Research & Development. Variety of conferences & seminars are conducted to update industry news and knowledges. Business networking activities, business matching, buyer privilege program will be held along with the exhibition. The exhibition will take place at BITEC, in co-location with Bio Asia Pacific and FutureCHEM INTERNATIONAL. Organized by VNU Asia Pacific

Mark your calender and see you soon!
Do look forward to our novelty goods and other surprises.

For more information and details, please contact us.

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SHODEX – 6th Edition Lab Indonesia (2022)

event | 07 August 2022
Title:

6th Edition Indonesia Laboratory, Scientific Analytical Equipments and Services Exhibition and Conference.

Venue:

Jakarta Convention Center (JCC), Jakarta – Indonesia

Date:

7 – 9 September 2022 | 10.00 am – 06.00 pm

Booth No.:

G 02

Tag: food and beverage Indonesia Pharmaceutical Shodex

Shodex Singapore (Booth No. G-02) will join 6th Edition Indonesia Laboratory, Scientific Analytical Equipments and Services Exhibition and Conference.

Lab Indonesia 2022 will be held at Jakarta Convention Center (JCC) Jakarta, Indonesia from 7 to 9 September 2022.

We will also conduct Technical Seminar on the third day,

Date: Friday, 9 September 2022

Location: Seminar Room 2

Title: Fundamental of HPLC Theory and Pharmaceutical Applications.

Speaker: Mr Tomokazu UMEZAWA

Time: 14:50 – 15:35

Food and beverage, industrial application customers also welcome!

Mark your calender and see you soon!
Do look forward to our novelty goods and other surprises.

For more information and details, please contact us.

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SHODEX Chromato News July 2022

news | 14 July 2022
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Effects of Eluent Composition Ratios on PFOS and PFOA Analysis (RSpak JJ-50 2D) >> Application Note

Polyamide (18) Nylon 6(3)T (GPC HK-404L) >> Application Note

Polyamide (17) Nylon 6/12 (GPC HK-404L) >> Application Note

Polyamide (16) Nylon 6/6 (GPC HK-404L) >> Application Note

Polyamide (15) Nylon 6/10 (GPC HK-404L) >> Application Note

Polyamide (15) Nylon 12 (GPC HK-404L) >> Application Note

Polystyrene Standards (26) (GPC HK-403) >> Application Note

Polystyrene Standards (25) (GPC HK-402) >> Application Note

Polystyrene Standards (24) (GPC HK-401) >> Application Note

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SHODEX Chromato News June 2022

news | 14 June 2022
Tag: chromatonews shodex

NEW! Technical Article No. 20

SEC Multi-Faceted Analysis of Exosome (EV) Combination of UV, Florescence, and Light Scattering Detectors

Exosome, an extracellular vesicle (EV), is responsible for transmitting various information and substances in living organisms. EVs are receiving great interests in the fields of new modality, because of their potential uses as highly sensitive diagnostic markers and therapeutic applications. Their transmission and target specific characteristics make them potentially significant in the treatment of various diseases. EVs have unique common physical and physiological properties including their sizes and surface antigens. However, their complex cell generation processes create diversities in the properties and encapsulated substances. Thus, they are often treated as mixtures of nano vehicles with certain variations. Therefore, the manufacturing of EV as a biopharmaceutical via bioprocessing requires development of production and evaluation methods that ensures consistent reproduction of EVs with the same characteristics throughout basic research, proof of concept, all clinical trial stages and actual production for clinical use. In addition, it is also important to control impurities generated during the production procedures. Those impurities include cell-originated components with which also have diversities.

Shodex offers hydrophilic aqueous polymer-base SEC columns that are suitable for analyzing various biological components. Shodex SEC columns with various pore sizes cover a wide molecular weight range, even the large nanoscale objects including EVs. Especially, SB-806 HQ with its sufficient pore size holds and separates EV-class nanoscale objects (50-200nm). Combined with a variety of detectors, this is a useful comprehensive analysis tool for a complex bio-nano compounds preparation process. This technical article introduces an example of multifaceted analysis and monitoring of an EV sample purification process using a SEC column, Shodex OHpak SB-806 HQ, with 3 different detectors.

EV Samples at each purification step:
✓ cell cuture supernatant,
✓ concentrate,
✓ purified product.

Click here to read the full PDF version.

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SHODEX Chromato News May 2022

news | 14 May 2022
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Absolute Molecular Weight Determination of PMMA (LB-802.5) >> Application Note

☆ Analysis of Ferric Carboxymaltose (SB-804 HQ + SB-802.5 HQ) >> Application Note

Pullulan Standards (9) (GF-7M HQ) >> Application Note

Analysis of 10 – 50mer Oligo-DNAs (VN-50 2D) >> Application Note

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SHODEX Chromato News April 2022

news | 14 April 2022
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Analysis of Various Oligonucleotides by LC/UV/MS (VN-50 2D) >> Application Note
   1. Synthesized oligo-DNA 20mer(crude)
   2. Synthesized phosphorothioated oligo-DNA 20mer(crude)
   3. Synthesized oligo-RNA 20mer(cart
ridge purified)
   4. Synthesized phosphorothioated oligo-RNA 20mer(cartridge purified)

☆ Analysis of 2′-OMe and 2′-MOE Modified Phosphorothioated Oligo-RNA by LC/UV/MS (VN-50 2D) >> Application Note
   1. Synthesized phosphorothioated oligo-2′-MOE-RNA 20mer(cartridge purified)
   2. Synthesized phosphorothioated oligo-2′-MOE(1-5/16-20)-RNA 20mer(cartridge purified)
   3. Synthesized phosphorothioated oligo-2′-OMe-RNA 20mer(cartridge purified)
   4. Synthesized phosphorothioated oligo-RNA 20mer(cartridge purified)

★ Analysis of Ellagic Acid in a Supplement (C18U 2D) >> Application Note

☆ Quantification of Purified Stevia Extract (VG-50 4E) >> Application Note
   1. Rubusoside 

   2. Dulcoside A
   3. Stevioside
   4. Rebaudioside C 
   5. Rebaudioside A

★ Analysis of Semaglutide (KW-802.5) >> Application Note

☆ Analysis of Polyhydroxybutyric Acid (LF-804) >> Application Note
    *biodegradable plastic

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SHODEX Chromato News March 2022

news | 14 March 2022
Tag: chromatonews shodex

NEW! Technical Article No. 18

Analysis of Functional Ingredients (Anti-Obesity Agents) in Beverages and Foods

In Japan, since April 2015, the nation started the “Foods with Functional Claims (FFC)” system to help general consumers obtain correct information and wisely select foods. A FFC product package clearly shows that it is a FFC product and describes its claimed functionalities. FFC can be obtained by submitting the necessary documents to the consumer affairs agency. The submission does not require any national examination to prove ingredient’s safety nor functionality. FFC is approved, as long as the producer states the functionality of the food based on scientific evidences and be responsible to the statement. In March 2021, there are more than 3,500 submitted FFC. It can be said that the FFC market is keep expanding.

In this application, we optimized analytical conditions for the analyses of functional food ingredients – especially focused on anti-obesity agents that are registered as functional food ingredients in the database and which are used in commercial beverages and foods.

acetic acid in vinegar drinks,
catechins in green teas,
chlorogenic acid in coffee,
lactoferrin in a supplement, and
✓ α-linolenic acid in a cooking oil,

Click here to read the full PDF version.

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SHODEX Chromato News February 2022

news | 14 February 2022
Tag: chromatonews shodex

NEW! Technical Article No. 17

Rapid Analysis of Sugars, Organic Acids, and Alcohols

Bioethanol production process requires frequent rapid analyses of sugars, organic acids, and alcohols to monitor the proper operations of biomass fermentation steps. There are seven main target analytes: two maltooligosaccharides, maltotriose and maltose, its constituent sugar, glucose, and the final products, ethanol, lactic acid, acetic acid, and glycerol.

In this work, we used a Shodex™ SUGAR™ SH1011 8C for the analysis of those seven target components. Shodex™ SUGAR™ SH1011 8C is a 10-cm rapid analysis column filled with rigid styrene-divinylbenzene copolymer base material. The strong cation exchange gel works to provide a mixed mode of size exclusion and ion exclusion chromatography, which is suitable for simultaneous analysis of various sugars and organic acids. The analysis time of Shodex™ SUGAR™ SH1011 8C is one thirds of our regular 30-cm analysis column, Shodex™ SUGAR™ SH1011. Thus, it is well suited for rapid analyses of sugars and organic acids.

maltotriose,
maltose,
glucose,
ethanol,
lactic acid,
acetic acid, and
glycerol.

Click here to read the full PDF version.

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New SHODEX™ Catalog 2022 Ver. for Download

news | 21 January 2022
Tag: shodex

Looking for the latest updated SHODEX™ catalog? You can view and download the newest 2022 version here.

Learn more about SHODEX™ HPLC columns in our product page. (Catalog, Application data, Operation Manuals)

Please feel free contact us for technical and sales support!

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SHODEX Chromato News January 2022

news | 14 January 2022
Tag: chromatonews shodex

NEW! Technical Article No. 16

Evaluating the Freshness of Fish Flesh Using the K Value

The K value is an index for estimating the freshness of fish flesh. The amount of adenosine 5’-triphosphate (ATP) in fish flesh is calculated from a method developed in 1959. Since endogenous enzymes degrade ATP in dead seafoods rapidly, the K value can be a quantitative measurement of seafoods’ freshness.

We developed an HPLC method to separate and quantify six ATP related substances in fish flesh extracts. The column used was Shodex™ Asahipak™ GS-320 HQ, a multimode column. The GS-320 HQ provides combinations of several separation modes, such as size exclusion, reversed phase, and ion exchange modes, in different degrees by varying the mobile phases. Using this unique feature, nucleic acid and ATP related substances can be separated under isocratic elution methods.

In this technical article, we focused on following three points to show the effectiveness of Shodex™ Asahipak™ GS-320 HQ for the analysis of ATP related substances.
1. To be able to separate six ATP related substances.
2. To be able to obtain calibration curves, suitable for quantification.
3. The method is not negatively influenced from the sample matrix

✓ adenosine 5′-triphosphate (ATP) dipotasium hydrate,
✓ adenosine 5′-diphosphate(ADP) disodium hydrate,
✓ adenosine monophosphate (AMP),
✓ disodium5′-Inosinate (IMP),
✓ inosine (HxR), and
✓ hypoxanthine (Hx)

Click here to read the full PDF version.

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SHODEX Chromato News December 2021

news | 14 December 2021
Tag: chromatonews shodex

NEW! Technical Article No. 15

Purification and SEC/MS Analysis of Norovirus Virus-Like Particles

In the fields of new modality, such as gene therapy drugs, virus vector, new vaccine, and drug delivery etc., applied researches on “bionanoparticles” are rapidly expanding and receiving the greatest interest these days. Due to their complex structures and distinct sizes, production and quality evaluation of their biological processes require integrated and multifaceted analytical techniques, even more than that of conventional biopharmaceutical compounds.

Virus-like particles (VLPs) are typical bionanoparticles. Since they are not infectious nor proliferated, but they can reproduce their original sizes and forms, they effectively elicit immune responses when used for vaccine. R&D for its commercialization is in progress as it would be an excellent platform.

This article presents the effectiveness of Size Exclusion Chromatography (SEC) – Shodex SB-805 HQ – for the analysis of bionanoparticles. As a proof of concept, method development for a chromatographic purification of norovirus cell surface layer binding protein originated VLP (NVLP) is discussed. This article also includes the evaluation of an effective method development using an SEC column with different analytical devices and a summary of how to select a suitable column for the monitoring of purification procedures.

Click here to read the full PDF version.

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SHODEX – Indonesia Webinar #4: Theory of LEX and SUGAR Column Applications

event | 18 November 2021
Title:

4th SHODEX Webinar with PT Batuwaris Dinamika (in Bahasa Indonesia)

Venue:

ZOOM

Date:

30th November 2021 | 13.30 – 14.30 WIB

Tag: Indonesia Shodex

Shodex SUGAR series allow the separation of monosaccharides and disaccharides using aqueous eluent under ligand exchange and GFC modes. Basically, saccharides are eluted in order of decreasing molecular weight by GFC mode. The series provides base line stability and linearity over a wide range when quantifying samples, thus permitting the detection of refractive indexes with high sensitivity. Ligand exchange mode is a separation mode, which employs ion interaction between metal ions (M+) which are the counter ions of the packing material, and hydroxyl (OH-) groups of the saccharides. Find out about the basic principles by joining this webinar. We will also introduce some application data that might be useful for you. 

Presentation will be conducted in Bahasa Indonesia.
Presentasi akan dihantarkan dalam Bahasa Indonesia.

Click here for registration. (Approx. 1 minute)

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SHODEX Chromato News November 2021

news | 14 November 2021
Tag: chromatonews shodex

NEW! Technical Article No. 14

Analysis of Functional Ingredients in Dietary Supplements

In Japan, since April 2015, the nation started the “Foods with Functional Claims (FFC)” system to help general consumers obtain correct information and wisely select foods. A FFC product package clearly shows that it is a FFC product and describes its claimed functionalities. The product producers obtain the FFC by submitting the necessary documents to the Consumer Affairs Agency. The submission does not require any national examination to prove ingredient’s safety nor functionality. FFC is approved, as long as the producer states the functionality of the food based on scientific evidences and be responsible for the statement. There are more than 2,000 submitted FFC, this number is now more than the approved FOSHU. It can be said that the FFC market is keep expanding.

In this application, we optimized analytical conditions for the analyses of functional food ingredients that are registered as functional food ingredients in the database and which are used in commercial dietary supplementPolymers contain various additives for the additional functionality and stabilization of the product. Analysis of such additives is important for the quality control purposes in the polymer production. Those additives are typically analyzed by liquid chromatography and gas chromatography after pretreating the sample by extraction and concentration. The treatment steps are often complex and time consuming. On the other hand, by using the gel permeation chromatography (GPC), a polymer sample dissolved in an appropriate solvent can directly be analyzed. It separates the additives from polymers, and thus a simple and rapid detection can be expected.

✓ unsaturated fatty acids (docosahexanenoic acid (DHA), eicosapentaenoic acid (EPA))
✓ amino acid (Theanine)
✓ choline derivative (L-α-Glycerylphosphorylcholine (α-GPC))
salacinol and kotalanol 
coenzyme Q10

Click here to read the full PDF version.

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SHODEX Chromato News October 2021

news | 14 October 2021
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Analysis of Oligo-DNAs (VN-50 2D) >> Application Note

☆ Analysis of Maltodextrin (VN-50 4D) >> Application Note

★ Analysis of Hexavalent Chromium by Post-Column Absorptiometry (SI-50 4E) >> Application Note

☆ Separation of Fosfomycin and its thermal decomposition products (NH2P-50 4E) >> Application Note

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SHODEX – Lab Indonesia Digital Exhibition Oct 2021

event | 06 October 2021
Title:

The 2nd Digital Business Networking for Lab Technology in Indonesia

Venue:

Online (web-based via GRIP platform)

Date:

27- 29 October 2021 ‪| 10.00am – 6.00pm‬ (GMT+7, Jakarta Time)

Tag: food and beverage Indonesia Pharmaceutical Shodex

Shodex Singapore will join Lab Indonesia’s 2nd digital business networking for lab technology. The three days event is a web-based hypermedia with high – tech online system as well as a platform that allows industry professionals from lab industry to engage, interact and connect digitally.

Date : ‪27 – 29 October 2021‬
Day ‪ : Wednesday – Friday

Time : ‪10.00am – 6.00pm‬ (GMT+7 Jakarta Time)

Visitors may register here to get your access to Lab Indonesia Digital Business.
Stay update and gain knowledge directly through PC, Tablet, or handphone with instant meeting.

Click here for seminar schedule by industry associations and government bodies.

Mark your calender and see you soon!

For more information and details, please contact us.

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SHODEX: Better Multivitamins Analysis with Polymer Columns ODP, NH2P

news | 20 September 2021
Tag: indonesia shodex thailand vitamin e

Are you seeking better column lifetime than C18 for Vitamin A, Vit D, Vit E, Vit K analysis?
Or not satisfied with separation by reversed phase for Vitamin B and Vit C analysis?

Shodex™ is a specialist for high-quality polymer-based columns with long lifetime (Reversed Phase RP, HILIC, SUGAR, GPC). In This article we’d like to introduce our Reversed Phase and HILIC Polymer column for multivitamin analysis application.

1) Fat-Soluble Vitamins
Asahipak ODP-50 4E [USP L67] reversed phase mode was used for the simultaneous analysis of fat-soluble vitamins. Good calibration curves were obtained for them.

Sample : 20 μL
1. Vitamin K3 1.5μg/mL
2. Vitamin A 1.0IU/mL(0.3μg/mL)
3. Vitamin A acetate 0.5IU/mL(1.9μg/mL)
4. Vitamin D2 13.2IU/mL(0.3μg/mL)
5. Vitamin D3 13.2IU/mL(0.3μg/mL)
6. Vitamin E acetate 2.4μg/mL
7. Vitamin E 2.5μg/mL
8. Vitamin K1 2.4μg/mL

Column : Shodex Asahipak ODP-50 4E (4.6 mm I.D. x 250 mm) 
Eluent : CH3CN/CH3OH = 50/50
Flow rate : 0.6 mL/min
Detector : UV (280 nm)
Column temp. : 30 °C

2) Food Containing Vitamins
Vitamins in food were analyzed using RSpak DE-413 [USP L71), a column for reversed phase chromatography.

(Sample pretreatment)
– Dissolve 80mg of the food containing vitamins in 12mL of the eluant (A).
– Pass through a 0.45μm filter before injecting into HPLC.

Sample : 20 μL
1. Vitamin C 145μg/mL
2. Vitamin B6 21.2μg/mL
3. Vitamin B2 7.3μg/mL

Column : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm) 
Eluent : (A); 1/30M Potasium phosphate buffer (pH 6.8) / CH3CN = 98/2
               (B); H2O / CH3CN = 50/50
                Gradient: 0 min to 4 min, 100% (A), 4 min to 15 min, 100% (A) to 30% (A), After 15 min, 30% (A)
Flow rate : 0.6 mL/min
Detector : UV (254 nm)
Column temp. : 35 °C

3) Water-Soluble Vitamins
To those who are not satisfied with RP mode for water-soluble vitamins, NH2P-50 4E [USP L82] offers ion exchange chromatography. Six water-soluble vitamins were separated within 5 minutes.

[Note] This analysis requires column equilibration.
For column equilibration: First introduce 60 mM phosphoric acid at 0.5 mL/min for 2 hours, and then switch to the eluent.

Sample : 20 μL
1. Vitamin B6 50μg/mL
2. Nicotinamide 10μg/mL
3. Vitamin B12 10μg/mL
4. Nicotinic acid 10μg/mL
5. Folic acid 10μg/mL
6. Vitamin C 10μg/mL

Column : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) 
Eluent : 40 mM H3PO4 aq./CH3CN = 45/55
Flow rate : 1.0 mL/min
Detector : UV (254 nm)
Column temp. : 40 °C

Please do not hesitate to contact us for further information and demo column availability.

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SHODEX Chromato News September 2021

news | 14 September 2021
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Analysis of Urea, an Impurity in Fluorouracil Injection Proposed in USP-NF Pharmacopeial Forum (SB-802.5 HQ) >> Application Note

☆ Analysis of Enoxaparin Sodium (KW-803 + KW-802.5) >> Application Note

★ Analysis of Meglumine (YS-50) >> Application Note

☆ Analysis of Meglumine (NN-814) to replace NN-414 >> Application Note

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SHODEX Chromato News August 2021

news | 15 August 2021
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Analysis of 2-Deoxy-D-glucose (VG-50 4E)(add 2-Glucal) >> Application Note

☆ Biodegradable Polymer (6) (Polylactic Acid) (KF-806M) >> Application Note

Citric Acid Analysis in Functional Beverage (KC-811) >> Application Note

Carotene Analysis in Carrot Juice (C18U 2D) >> Application Note

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SHODEX: Analyse Azithromycin with ODP-50 4E According to USP

news | 10 August 2021
Tag: indonesia shodex

Tempo.co news (15 July 2021) reported the Indonesian Food and Drug Monitoring Agency or BPOM has finally granted the Emergency Use Authorization for anti-parasitic drug ivermectin as the therapeutic drug to support the Covid-19 treatment. […] The Circular was inked by the BPOM head No. HK.02.02.1.2.07.21.281 of 2021 on the second amendment to the BPOM Decree No. HK.02.02.1.2.11.20.1126 of 2020. Besides ivermectin, the circular noted seven other therapeutic drugs for Covid-19 treatment, viz. Remdesivir, Favipiravir, Oseltamivir, Immunoglobulin, Ivermectin, Tocilizumab, Azithromycin, and Dexamethasone.

Azithromycin is a 15-membered ring macrolide antibiotic. According to the United States Pharmacopeia and the National Formulary (USP 39-NF 34**), analysis of azithromycin should be carried out with a column filled with L67 packing material and meets following requirements. The Asahipak ODP-50 4E confirmed the requirements were met.
System suitability requirements:
 Resolution of azithromycin and azaerythromycin A: ≥ 3.0
 Relative standard deviation (RSD): ≤ 1.1%
 Tailing factor of azithromycin: 0.8 to 1.5
**The version at the time of the application acquisition.

Sample : 10 μL
Azithromycin
Azaerythromycin A
dissolved in 6.7 g/L Dibasic potassium phosphate aq. (pH8.0 adjusted with phosphoric acid)/CH3CN=40/60

Column : Shodex Asahipak ODP-50 4E (4.6 mm I.D. x 250 mm)
Eluent : 6.7 g/L Dibasic potassium phosphate aq. (pH11.0 adjusted with 10 M KOH) /CH3CN=40/60
Flow rate : 1.0 mL/min
Detector : UV (210 nm)
Column temp. : 40 °C

Source – (2021, July 15). BPOM Approves Ivermectin as Covid-19 Therapeutic Drug. Tempo.co. https://en.tempo.co/read/1483423/bpom-approves-ivermectin-as-covid-19-therapeutic-drug 

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SHODEX: Analyse 2-Deoxy-D-glucose (2-DG) with VG-50 4E

news | 22 July 2021
Tag: india shodex

BBC News (7 May 2021) reported that India has begun distributing a new locally developed drug to treat Covid-19 symptoms.
Authorities have approved 2-DG and it is to be first used in hospitals across the capital, Delhi. According to a government statement, it “will be of immense benefit to the people suffering” from Covid-19.

2-DG or 2-Deoxy-D-glucose is a glucose molecule which has the 2-hydroxyl group replaced by hydrogen, and it is not metabolized by glycolysis. D-Glucal is the starting material for the synthesizing of 2-Deoxy-D-glucose. The separation of 2-Deoxy-D-glucose, D-Glucal and glucose was made by Shodex HILICpak VG-50 4E column which is a polymer-based amino column.

Sample : 10 µL
0.15 %(w/v) each in H2O/CH3CN=5/5
1. D-Glucal
2. 2-Deoxy-D-glucose
3. Glucose

Column : Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm)
Eluent : H2O/CH3CN=12/88
Flow rate : 1.0 mL/min
Detector : RI
Column temp. : 40 ℃

Source – (2021, May 7). India rolls out new local Covid drug after emergency approval. BBC News. https://www.bbc.com/news/world-asia-india-57139919 

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SHODEX Chromato News July 2021

news | 14 July 2021
Tag: chromatonews shodex

New Application Data on Shodex.com

Epoxy Resin (6) (Comparison of HK-402 and KF-802.5) >> Application Note

Epoxy Resin (7) ( Effects of Flow Rate for HK-402) >> Application Note

★ SEC/MALS Analysis of Exosome (SB-806 HQ) >> Application Note

Steviol Purity Test of Purified Stevia Extracts According to Japanese Pharmaceutical Excipients Method (C18M 4E) >> Application Note

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SHODEX Chromato News June 2021

news | 15 June 2021
Tag: chromatonews shodex

NEW! Technical Article No. 13

GPC/MS Analysis of Polymer Additives

Polymers contain various additives for the additional functionality and stabilization of the product. Analysis of such additives is important for the quality control purposes in the polymer production. Those additives are typically analyzed by liquid chromatography and gas chromatography after pretreating the sample by extraction and concentration. The treatment steps are often complex and time consuming. On the other hand, by using the gel permeation chromatography (GPC), a polymer sample dissolved in an appropriate solvent can directly be analyzed. It separates the additives from polymers, and thus a simple and rapid detection can be expected.

Shodex™ Technical Article No. 8: “Ultra-Rapid Analysis of High Molecular Weight Compounds Using SEC Mode” summarized the ultra-rapid analysis of high molecular weight compounds using Shodex™ GPC HK-400 series columns, which are packed with uniformly controlled 3- or 3.5-µm styrene divinylbenzene copolymers. This article introduces the rapid, highly sensitive, and highly selective applications analyzing various polymer additives using HK-400 series columns with THF eluent and an MS detector.

✓ phenolic antioxidants (Irganox 1010, Irganox 1076)
✓ hindered amine light stabilizer/HALS (ADK STAB LA-68).

Click here to read the full PDF version.

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SHODEX – Indonesia Webinar #3: Introduction and Application of SEC (GPC/GFC)

event | 03 June 2021
Title:

3rd SHODEX Webinar with PT Batuwaris Dinamika (in Bahasa Indonesia)

Venue:

ZOOM

Date :

22th June 2021 | 13.00 – 14.00 WIB

Tag: Indonesia Shodex

Size Exclusion Chromatography (SEC) is completely different from other chemical/ionic separation modes as it using analytes’ physical characteristics. Find out about the basic principles by joining this webinar. We will also introduce some application data that might be useful for you. 

Presentation will be conducted in Bahasa Indonesia.
Presentasi akan dihantarkan dalam Bahasa Indonesia.

Click here for registration. (Approx. 1 minute)

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SHODEX – The 6th Edition of Lab Indonesia 2021 Rescheduled to October’21

event | 18 May 2021
Tag: food and beverage Indonesia Pharmaceutical Shodex

After careful deliberation with key industry members, it is decided the 6th Edition of Lab Indonesia reschedule to 27-29 October 2021, at the same venue, Jakarta International Expo, Kemayoran.  

Despite COVID-19 situation, Shodex Singapore team are still commited to maintain our support as much as possible for customers.
We also started YouTube channel to share our knowledge with you for free. We hope they are useful for your day-to-day tasks.
Some of the videos are also displayed in the Shodex product page here.

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SHODEX Chromato News May 2021

news | 15 May 2021
Tag: chromatonews shodex

NEW! Technical Article No. 12

Simultaneous LC/MS Analyses of Phosphorylated Saccharides

Phosphorylated saccharides take the important role as intermediates in various metabolic pathways of animals. For example, during glycolysis process, glucose is first converted to glucose-6-phosphate, then to other phosphorylated saccharides (such as fructose-6-phosphate and fructose-1,6-bisphosphate) and finally to pyruvate to produce ATP, the energy source of cell processing. Through glycogenolysis process, glucose-1-phosphate is produced from breakdown process of glycogen branches. In this process, glucose-6-phosphate is also produced as an isomer, and the product goes through a glycolysis process via different pathway. High sensitive analysis of phosphorylated saccharides in vivo is important for understanding the biological phenomena and early detection and treatment of the related diseases. One such case is glycogenosis, which stores abnormal amounts of glycogen in the body, and is possibly related to genetic glycogen enzymes deficiencies. Newborn glucose-6- phosphate concentration monitoring is considered an effective tool for the early detection of the disease.

In this application, we used a combination of Shodex™ HILICpak™ columns and LC/MS for simultaneous and high sensitive analysis of various phosphorylated saccharides without the use of ion-pair reagent nor derivatization.

✓ Phosphorylated Saccharides Using HILICpak™ VT-50 2D (glucose-1-phosphate (G1P), glucose-6-phosphate (G6P), fructose-1-phosphate (F1P), fructose-6-phosphate (F6P), fructose-1,6-bisphosphate (F1,6bP), and uridine diphosphate glucose (UDP-Glc));
✓ Phosphorylated Saccharides Using HILICpak™ VG-50 2D (fructose, glucose, G1P, G6P, UDP-Glc, and F1,6bP)

Click here to read the full PDF version.

Contact us today for your free, no-obligation technical consultation and demo offer.

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SHODEX Chromato News April 2021

news | 15 April 2021
Tag: chromatonews shodex

NEW! Technical Article No. 11

HPLC Analysis of Antidiabetic Drugs

Drugs, including antidiabetic drugs, with different targets have various chemical structures. This makes it difficult for an HPLC method to simultaneously analyze all antidiabetic drugs which may contain peptides, sugars, and lowmolecular weight compounds of different properties. Thus, analysis of each drug requires a specific method with an appropriate column and optimized analytical conditions. This technical article introduces application data for the analysis of four antidiabetic drugs using Shodex™ columns.

✓ Oral Antidiabetic Drug (Metformin);
Insulin
Liraglutide; and
Exenatide

Click here to read the full PDF version.

Contact us today for your free, no-obligation technical consultation and demo offer.

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SHODEX – New Video Release “How to analyze Hydrophilic Substance using HPLC”

news | 19 March 2021
Tag: shodex

Click the here to view our latest video on  Shodex Singapore YouTube Channel.

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SHODEX Chromato News March 2021

news | 15 March 2021
Tag: chromatonews indonesia shodex thailand

New Application Data on Shodex.com

★ Analysis of Thymosin α1 (KW-802.5) >> Application Note 

☆ Analysis of GABA Supplement (NH2P-50 4E) >> Application Note 

★ SEC Analysis of Latex Particles (KW405-4F) >> Application Note 

☆ Effects of Organic Solvent Concentration on HILIC Mode (NH2P-50 4B) >> Application Note 

★ Effects of Eluent pH on HILIC Mode (VG-50 2D) >> Application Note

Contact us today for your free, no-obligation technical consultation and demo offer.

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SHODEX Chromato News February 2021

news | 15 February 2021
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Analysis of Zinc Sulfate Compounded Injection According to USP-NF Method (YK-421) >> Application Note

☆ Analysis of Potassium Bicarbonate and Potassium Chloride Effervescent Tablets for Oral Solution Proposed in USP-NF Pharmacopeial Forum (YK-421) >> Application Note

★ Analysis of Potassium and Sodium Bicarbonates and Citric Acid Effervescent Tablets for Oral Solution Proposed in USP-NF Pharmacopeial Forum (YK-421)  >> Application Note

☆ Analysis of Betadex Sulfobutyl Ether Sodium According to USP-NF Method (SB-803 HQ) >> Application Note

★ Analysis of Sucrose Proposed in USP-NF Pharmacopeial Forum (SC1011-7F) >> Application Note

☆ Analysis of Propylene Glycol Monocaprylate According to USP-NF Method (KF-801) >> Application Note

Contact us today for your free, no-obligation technical consultation and demo offer.

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SHODEX Chromato News January 2021

news | 20 January 2021
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Analysis of Lactoferrin Supplement (C4P-50 4D)
☆ Analysis of Lactoferrin Concentrate According to Analytical Methods of Food Additives in Foods (C4P-50 4D)
★ Analysis of Erythrose and Threose (SC1011)
☆ Analysis of Erythrose and Erythritol (VG-50 4E)
★ Analysis of α-Linolenic Acid in Edible Blended Oil (ODP-50 4D)
☆ Analysis of Pamidronate Disodium According to USP-NF Method (IC I-524A)
★ Analysis of Sodium Risedronate (IC I-524A)
☆ Analysis of Sodium Ibandronate (IC I-524A)
★ Analysis of Zoledronic Acid (IC I-524A)

Click here to read the full PDF version >>> Chromato News JAN 2021 – English

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SHODEX – New Video Release “5 Things You Should Do Before Sample Analysis in HPLC”

news | 11 December 2020
Tag: shodex

Click the here to view our latest video on  Shodex Singapore YouTube Channel.

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SHODEX Chromato News November 2020

news | 30 November 2020
Tag: chromatonews shodex

New Application Data on Shodex.com

★ Analysis of Oligomers in Omega-3 Free Fatty Acids According to USP-NF (KF-802.5 + KF-802 + KF801)
☆ Analysis of Calcium Acetate Capsules According to USP-NF (YK-421)
★ Analysis of Chloride According to the Japanese Testing Methods for Fertilizers (NI-424)
☆ Analysis of Chloride According to the Japanese Testing Methods for Fertilizers (SI-52 4E)
★ Analysis of Levocarnitine (NH2P-50 4E)
☆ Rapid Analysis of Malto-oligosaccharides, Organic Acids, and Ethanol (SH1011 8C)
★ Analysis of Inositol According to Japanese Pharmaceutical Excipients 2018 (KS-801)

Click here to read the full PDF version >>> Chromato News NOV 2020 – English

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SHODEX – New Video Release: “Understand HILIC mode in HPLC”

news | 23 October 2020
Tag: food and beverage shodex

Click the here to view our latest video on  Shodex Singapore YouTube Channel.

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SHODEX Chromato News October 2020

news | 21 October 2020
Tag: chromatonews shodex

Analysis of Functional Sugars in Foods Using HILIC Mode.

Recently, the effects of functional sugars in foods are gaining scientists’ interests, and thus their analytical methods are called for. This article will demonstrate the effectiveness of Shodex™ HILICpak™ VG-50 4E with its HILIC mode for the analysis of various functional sugars in foods. The eluent typically used in HILIC mode is a mixture of water and acetonitrile. And for some applications, the separation efficiency of the VG-50 4E can be improved by using a mixture containing water, acetonitrile, and methanol. We also proved the effectiveness of the developed method for analyzing functional sugars in real food products.

Click here to read the full PDF version >>> Chromato News OCT 2020 – English

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SHODEX – Lab Indonesia Digital Exhibition Nov 2020

event | 20 October 2020
Title:

The 1st Digital Business Networking for Lab Technology in Indonesia

Venue:

Online (web-based via GRIP platform)

Date:

25- 27 November 2020 ‪| 10.00am – 6.00pm‬ (GMT+7 Jakarta Time)

Tag: food and beverage Indonesia Pharmaceutical Shodex

Shodex Singapore will join Lab Indonesia’s 1st digital business networking for lab technology. The three days event is a web-based hypermedia with high – tech online system as well as a platform that allows industry professionals from lab industry to engage, interact and connect digitally.

Day ‪ : Wednesday – Friday‬
Date : ‪25 – 27 November 2020‬
Time : ‪10.00am – 6.00pm‬ (GMT+7 Jakarta Time)

Visitors may register here to get your access to Lab Indonesia Digital Business:
http://www.pamerindo-eob.com/aplikasi/labindonesiaform/

Don’t forget to mark your calender! For more information and details, please contact us.

See you!

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SHODEX: Polymer-based HPLC Column Free Demo Offer!

news | 06 October 2020
Tag: food and beverage shodex

Are you facing durability issue with your HPLC column?

By P.V.

Repeating analysis for multiple times sound miserable for the researchers, especially when we are busy with many projects and approaching deadlines.

“HPLC is High Performance Liquid Chromatography, a chromatographic technique used to split a mixture of compounds in the fields of analytical chemistry, biochemistry and industry. ”
The main purposes for using HPLC are to identify and quantify each component in the mixture.
And, HPLC column is one of the most important parts for HPLC system to get you the right result.

Silica columns have been used in conventional HPLC analysis, but nowadays customers’ needs are diversifying, polymer columns with following characteristics are receiving more attention.

Features of polymer column:

  • wide pH range (2-13)
  • possibility to use in low salt concentration
  • good separation of hydrophilic substances
  • no-silanol groups, so no chemical interactions which is cause of tailing peak

Contact us to request free demo of Shodex polymer column for you your trialing purpose.
*T & C Applies

Showa Denko has developed expertise in the analysis of polymers, pharmaceuticals, sugar, and proteins for 55 years. Based on this experience, we are developing the popular brand SHODEX ™ of HPLC polymer columns, which offers high quality and a wide range of applications, all over the world.

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SHODEX: Announcement of Change in YS-50 Shipping Solvent

news | 31 August 2020
Tag: shodex

Shodex will be changing the shipping solvents for the below listed product.
Please note that the actual shipping solvent used will be different from the shipping solvent described in our 2020-2021 ShodexTM Catalog.

Product
Ion Chromatography columns for cations
F7122000  IC YS-50
(Listed on Page 34 in the 2020-2021 ShodexTM Catalog)

Details About Change in Shipping Solvents
Present (catalog) : H2O
After change         : 10 mM Na2SO4 aq.

Effective Date
Products manufactured after September 2020

This news originally published on shodex.com

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SHODEX – Postponement Notice on The 6th Edition of Lab Indonesia 2020 to 2021

event | 26 August 2020
Title:

The 6th Edition of Lab Indonesia

Venue:

Jakarta International Expo, Kemayoran

Date:

1st – 3rd September 2021| 10.00 – 17.00 hrs

Booth No.:

TBA

Tag: food and beverage Indonesia Pharmaceutical Shodex

Due to the increasing number of cases worldwide and the travel uncertainty, the 6th Lab Indonesia which is scheduled to be held on 25-27 November 2020 at Jakarta International Expo, Kemayoran will be postponed to 1-3 September 2021.

Initially, Lab Indonesia 2020 was postponed from April to November to enhanced quality of the event, with more exhibitors and qualified trade buyers. However, in light of the uncertainties from COVID-19, which include travel restrictions, health & safety concerns as well as business climate. Therefore, with the interests of the event and its stakeholders, it’s decided to postpone again to September 2021. 

Despite COVID-19 situation, Shodex Singapore team are still commited to maintain our support as much as possible for customers.
We also started YouTube channel to share our knowledge with you for free. We hope they are useful for your day-to-day tasks.
Some of the videos are also displayed in the Shodex product page here.

Contact Us

SHODEX – New Video Release: “What is Reversed-phase Chromatography and Shodex HPLC Polymer-based column??”

news | 13 August 2020
Tag: shodex

Click the here to view our latest video on Shodex Singapore YouTube channel.

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SHODEX Chromato News August 2020

news | 01 August 2020
Tag: chromatonews shodex

New Application Data on Shodex.com!

★ LC/MS Analysis of Glyphosate and Glufosinate with (VT-50 2D)
☆ Charge Variant Analysis of Trastuzumab with (SP-FT 4A)
★ Charge Variant Analysis of Cetuximab with (SP-FT 4A)
☆ Charge Variant Analysis of Cetuximab (2) with (SP-FT 4A)
★ Speciation of Chromium by LC/ICP-MS (2) with (VC-50 2D)

Click here to read the full PDF version >>> Chromato News AUG 2020 – English

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SHODEX: Announcement of Change in SI-35 4D Shipping Solvent

news | 14 July 2020
Tag: shodex

Shodex will be changing the shipping solvents for the below listed product.
Please note that the actual shipping solvent used will be different from the shipping solvent described in our 2020-2021 ShodexTM Catalog.

Product
Ion Chromatography Columns for suppressor method (KOH Eluent)

F6999361     IC SI-36 4D
(Listed on Page 32 in the 2020-2021 ShodexTM Catalog)

Details About Change in Shipping Solvents
Present (catalog): 25 mM KOH aq.

After change       : 10 mM Na2SO4 aq.

Effective Date
Products manufactured after July 15th 2020

This news originally published on shodex.com

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SHODEX Chromato News July 2020

news | 01 July 2020
Tag: chromatonews shodex

Ultra-Rapid Analysis of Proteins by Cation Exchange Chromatography

High Performance Liquid Chromatography (HPLC) takes an important role in the pharmaceutical and biotech industry for the analysis of biological components such as proteins, peptides, and nucleic acids. The Shodex™ IEC SP-FT 4A shortens the analysis time to one-third of previous IEC SP-420N, while the column remains to provide sharp peak shapes. Dimensions are 4.6 mm inner diameter and 10 mm length, and housing made of PEEK.

Application data includes:

  1. Ovalbumin
  2. Trypsinogen
  3. Ribonuclease A
  4. Cytochrome C
  5. Lysozyme
  6. α-Chymotrypsinogen A
  7. AFSC Hemoglobin Control, normal hemoglobins,
    HbF(F) and HbA(A), abnormal hemoglobins, HbS(S) and HbC(C)
  8. HbA1c or glycohemoglobin

Click here to read the full PDF version >>> Chromato News JUL 2020 – English

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SHODEX Chromato News June 2020

news | 01 June 2020
Tag: chromatonews shodex

New Application Data on Shodex.com!

★ Analysis of Allantoin in a Commercial Disinfectant (VG-50 4E)
☆ LC/MS/MS Analysis of Glyphosate and Glufosinate (VT-50 2D)
★ SEC Analysis of Filgrastim (KW-803)
☆ Analysis of Ethylcellulose Dispersion Type B According to USP-NF (KF-802)
★ Analysis of Coenzyme Q10 in a Dietary Supplement (C18P 4D)

Click here to read the full PDF version >>> Chromato News JUN 2020 – English

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SHODEX: Announcement of Change in ODP2 HP-4D Theoretical Plate Number Specification

news | 25 May 2020
Tag: shodex

This is a notification of change in ODP2 HP-4D theoretical plate number (TPN) specification.
Thank you for your understanding and we apologize for any inconvenience this may cause.
Should you have any question or concerns, please contact us using the “Contact Us” form.

Product
Polymer-based Columns for Reversed Phase Chromatography
F7622002  ODP2 HP-4D

Detail of the Specification Change
Old TPN  : ≥13,000
New TPN: ≥10,000

Effective Date
Products produced after May 25th, 2020

This news originally published on shodex.com

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SHODEX: New Product – C18U series – ODS Columns for UHPLC

news | 09 March 2020
Tag: shodex

The New C18U

· UHPLC column (Maximum pressure: 100 MPa)
· Achieves high performance analysis with sub-2 μm particles
· Organic/inorganic silica hybrid particles provide excellent resolution
   and mechanical stability and improved alkali durability (from pH 1 to 12)
· Usable in 100 % water and buffer solution
· Fulfills USP L1 requirements

Semi-micro columns
F6654011  C18U 2B  (I.D. 2.0 x  50)
F6654012  C18U 2D (I.D. 2.0 x 150)
  Functional group: Octadecyl
  Particle size (μm): 1.9
  Carbon load (%)  : 20    *Containing 8 % for hybrid silica base material.
  Pore size (Å)         : 120
  Shipping solvent  : CH3CN

Guard column for C18 series
F6709801  EXP® cartridges: 3 Cartridge columns
F6709802  EXP® direct connect holder: One holder (2 titanium hybrid ferrules and 1 nut included)
EXP is registered trademark of Optimize Technologies, Inc.

This news originally published on shodex.com

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SHODEX Chromato News February 2020

news | 01 February 2020
Tag: chromatonews shodex

New Application Data on Shodex.com!

★ Quantification of N-Methylpyrrolidine in Cefepime for Injection According to USP Method (YK-421)
☆ Quantification of Alendronate Sodium Hydrate According to JP Method (DS-413)
★ Analysis of Phosphorothioated Oligo DNA (VN-50 2D)
☆ SEC Analysis of PEG-Asparaginase (SB-806 HQ)

Click here to read the full PDF version >>> Chromato News FEB 2020 – English

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SHODEX Chromato News January 2020

news | 01 January 2020
Tag: shodex

★☆Happy New Year 2020 ★☆
SHODEX Singapore team wishes you a Prosperous and Joyful year ahead.

Ultra Rapid Analysis of High Molecular Weight Compounds by SEC Mode

Shodex have worked on the development of ultra rapid analysis GPC columns: Shodex™ GPC HK series.
The newly developed columns successfully reduced the analysis time and the eluent consumption volume. This technical note introduces the characteristics and the application examples of the Shodex™ GPC HK series columns.

Click here to read the full PDF version >>> Chromato News JAN 2020 – English

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SHODEX Chromato News November 2019

news | 01 November 2019
Tag: chromatonews shodex

New Anion Analysis Column using KOH Eluent: Shodex IC SI-36 4D

★ Analysis of Anions (SI-36 4D)
☆ Effects of Potassium Hydroxide Concentration on Retention Time (1) and (2) (SI-36 4D)
★ Effects of Temperature on Retention Time (SI-36 4D)

New Application Data on Shodex.com!

★ Analysis of Unsaturated Fatty Acids in Supplements (ODP-50 4D)
☆ Analysis of Polysorbate 80 in Antibody Drugs (ODP2 HP-4D)
★ Drug Antibody Ratio Analysis of Antibody-Drug Conjugate (ODP2 HP-4B)
☆ Analysis of Somatropin According to EP Method (LW-803)

Click here to read the full PDF version >>> Chromato News NOV 2019 – English

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SHODEX Chromato News September 2019

news | 01 September 2019
Tag: chromatonews shodex

New Application Data on Shodex.com!

★ Analysis of Theanine in Supplement (NH2P-50 4E)
☆ Analysis of Fructooligosaccharides in Accordance to the Food System for Specified Health Use (VG-50 4E)
Deoxyribonucleotide (WA-624)

Tips
☆ How to maximize the life of Shodex SUGAR columns when analyzing in high temperature

Click here to read the full PDF version >>> Chromato News SEP 2019 – English

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SHODEX – Thailand Lab International 2019

event | 15 August 2019
Title:

9th International Trade Exhibition and Conference on Analytical Laboratory Equipment & Technology, Biotechnology & Life Sciences and Chemicals & Safety

Venue:

BITEC, Bangkok International Trade & Exhibition Centre, Bangkok, Thailand

Date:

25th – 27th September 2019 | 10.00 – 17.00 hrs

Booth No.:

TBA

Tag: Pharmaceutical Shodex Thailand

Thailand LAB INTERNATIONAL is organized by VNU Exhibitions Asia Pacific Co., Ltd. a joint venture between Jaarbeurs from the Netherlands and TCC Exhibition and Convention Center from Thailand. VNU Exhibitions Asia Pacific develops, with industry partners and exhibition organizers from around the world, executes and internationalizes trade show and conferences from animal production and processing to technology, from agriculture to innovations and from pets to medical and laboratory sectors. VNU Exhibitions Asia Pacific successfully applies its concepts and know-how across industries and in all Asian countries. Through its international network and global reach, VNU Exhibitions Asia Pacific builds marketplaces in Asia – for the world.

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SHODEX Chromato News August 2019

news | 01 August 2019
Tag: chromatonews shodex

New Application Data on Shodex.com!

Pullulan Standard Kit P-82 is now available for Indonesia
★ Separation of Water-Soluble Vitamin and Sulfite (KC-811)
Laminarin (SB-806M HQ)
★ Analysis of Epinephrine According to USP (In-Process Revision) (CDBS-453)
☆ Chiral Separation of Norepinephrine (CDBS-453)
Ginsenoside (Carrot Saponin) (NH2P-50 4E)
☆ LC/MS analysis of Polysorbate 20 in Antibody Drug Complex Preparation (ODP2 HP-2B)

Click here to read the full PDF version >>> Chromato News AUG 2019 – English

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SHODEX Chromato News July 2019

news | 01 July 2019
Tag: shodex

New Application Data on Shodex.com!

☆ Impurity Analysis of Voriconazole According to USP Method (CDBS-453)
★ Analysis of Theanine (NH2P-50 4E)
☆ SEC Analysis of Antibody Drug Conjugate (LW-803)
★ Impurity Analysis of Lamivudine According to USP Method (CDBS-453)
☆ SEC Analysis of Saccharated Ferric Oxide Injection (SB-804 HQ + SB-802.5 HQ)

Click here to read the full PDF version >>> Chromato News JUL 2019 – English

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SHODEX: Announcement of Discontinued Shodex Products

news | 07 June 2019
Tag: shodex

Shodex will discontinue the production of the products listed below at the end of 2019. We apologize for any inconvenience this may cause you.
Please contact us with questions about suitable replacement recommendations.
If you have any questions, please contact us from the “Contact Us” form.

Polymer-based Reversed Phase Chromatopraphy Columns

Product NameSize (mm)
I.D. x Length
ODP2 HP-3B3.0 x 50
ODP2 HP-3D3.0 x 150
ODP2 HPG-3A3.0 x 10
Asahipak ODP-40 3B3.0 x 50
Asahipak ODP-40 3D3.0 x 150
Asahipak ODP-40G 3A3.0 x 10
ODP40-2B2.0 x 50
ODP40-2D2.0 x 150
Asahipak C4P-50 10E10.0 x 250
Asahipak C4P-50G 7B7.5 x 50
Asahipak C8P-50 10E10.0 x 250
Asahipak C8P-50G 7B7.5 x 50
DE413-2B2.0 x 50
DE413-2E2.0 x 250
NN414-2D2.0 x 150
RSpak DE-201320.0 x 300
RSpak DE-G 8B8.0 x 50
RSpak DM-201420.0 x 300
RSpak DM-G 8B8.0 x 50

Polymer-based Hydrophilic Interaction Chromatography (HILIC) Columns

Product NameSize (mm)
I.D. x Length
Asahipak NH2P-40 3B3.0 x 50
Asahipak NH2P-40 3D3.0 x 150

Silica-based Reversed Phase Chromatopraphy Columns

Product NameSize (mm)
I.D. x Length
Silica 5PYE 4D4.6 x 150
Silica 5C8 10E10.0 x 250
Silica 5C8 20E20.0 x 250

Silica-based Normal Phase Chromatopraphy and Hydrophilic Interaction Chromatography (HILIC) Columns

Product NameSize (mm)
I.D. x Length
Silica 5SIL 10E10.0 x 250
Silica 5SIL 20E20.0 x 250
Silica 5NH 10E10.0 x 250
Silica 5NH 20E20.0 x 250

Ligand Exchange Chromatopraphy Columns

Product NameSize (mm)
I.D. x Length
RSpak DC-201320.0 x 300
RSpak DC-G 8B8.0 x 50

Ion Exclusion Chromatopraphy Column

Product NameSize (mm)
I.D. x Length
RSpak KC-201120.0 x 300

Multimode Columns

Product NameSize (mm)
I.D. x Length
Asahipak GS-620 20F20.0 x 300
Asahipak GS-620 20G20.0 x 500

Aqueous/Organic SEC Columns

Product NameSize (mm)
I.D. x Length
Asahipak GF-210 4E4.6 x 250
GF510A-4D4.6 x 150
GF510A-4E4.6 x 250
Asahipak GS-710 20F20.0 x 300
Asahipak GS-710 20G20.0 x 500
Asahipak GSM-700 20F20.0 x 300
Asahipak GSM-700 20G20.0 x 500

Origanic SEC (GPC) Columns

Product NameSize (mm)
I.D. x Length
GPC K-8078.0 x 300
GPC KF-6076.0 x 150
GPC HFIP-8078.0 x 300
GPC HFIP-6066.0 x 150
GPC HFIP-6076.0 x 150

Anion Exchange Chromatopraphy Columns

Product NameSize (mm)
I.D. x Length
IEC DEAE3N-4T4.6 x 35
PIKESS DEAE-2B2.0 x 50

Cation Exchange Chromatopraphy Columns

Product NameSize (mm)
I.D. x Length
IEC SP-420N4.6 x 35
PIKESS SP-2B2.0 x 50

This news originally published on shodex.com

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SHODEX Chromato News May 2019

news | 01 May 2019
Tag: chromatonews shodex

Wishing You A Blessed Ramadan
May the spirit of Ramadan show us the way to peace and harmony.

LC/MS Analysis of Glyphosate and Its Related Compounds Using Shodex™ HILICpakTM VT-50 2D

Organophosphate herbicides are globally used non-selective weed killer. Due to concerns on their toxicity to humans, monitoring their concentrations in environment and foods are important. In this edition, we show the analysis of 6 organophosphate herbicides: glyphosate and its metabolite, aminomethylphosphonic acid (AMPA), and glufosinate and its metabolites, 3-methylphosphinicopropionic acid (MPPA), ethephon, and fosetyl.

Click here to read the full PDF version >>> Chromato News MAY 2019 – English

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SHODEX Chromato News April 2019

news | 01 April 2019
Tag: chromatonews shodex

NEW! Technical Article No. 7

Analysis of Various Oligosaccharides by HILIC Mode

It is known that oligosaccharides are utilized by gut bacteria inside the animal bodies, providing digestive health benefits. HILIC mode are commonly used these days because it provides better separation for oligomers than SEC mode. Shodex™ HILICpak™ VN-50 series columns are specifically developed for the separation of high-molecular weight oligosaccharides that were difficult to be analyzed by previously available HILIC columns.

✓ Nonionic oligosaccharides such as dextrin, N-acetylchitooligosaccharides (polymer of N-acetylglucosamine) and xylooligosaccharides;
✓ Cationic oligosaccharides, chitosan-oligosaccharides (polymer of glucosamine); and
✓ Anionic oligosaccharides, oligogalacturonic acids

Click here to read the full PDF version >>> Chromato News APR 2019 – English

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SHODEX Chromato News March 2019

news | 01 March 2019
Tag: chromatonews shodex

New Applications Data on Shodex.com Website!

Methacrylic Acid Copolymer (KF-806M)
☆ Chiral Separation of Asparagine (CRX-853)
★ Chiral Separation of Proline (CRX-853)
☆ Chiral Separation of Tryptophan (CRX-853)
★ SEC Analysis of Liraglutide Formulation (KW-802.5)
☆ Analysis of Oligogalacturonic Acids (VN-50 4D)
★ LC/MS Analysis of Hexamethylenetetramine (VC-50 2D)
☆ Analysis of Sorbitol According to USP Method (SP0810 8C)
★ Analysis of Maltitol According to USP (SP0810 8C)
☆ Comparison of Sensitivity of NH2P Polymer-based Column and Silica-based Amino Column with ELSD

Click here to read the full PDF version >>> Chromato News MAR 2019 – English

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SHODEX Chromato News February 2019

news | 01 February 2019
Tag: chromatonews shodex

Shodex Singapore Team wishes You
🏮 Happy Lunar New Year! 恭喜发财🏮

NEW! Technical Article No. 6

Simultaneous Analysis of Ten Water-Soluble Vitamins Using a Polymer-Based Reversed Phase Column Shodex™ RSpak DE-413L

Methods using microbiological assays, absorption spectrophotometry, and HPLC are laborsome and time consuming process. Use of ODS column with an addition of ion-pair reagent tends to remain on the column and the flow-lines, results in increased background level and lowers the sensitivity. In this application, we aimed to develop a simple method to simultaneously analyze various water-soluble vitamins. We used Shodex™ RSpak DE-413L, a polymer-based reversed phase column, without a use of ion-pair reagent.

Click here to read the full PDF version >>> Chromato News FEB 2019 – English

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SHODEX Chromato News January 2019

news | 01 January 2019
Tag: chromatonews shodex

NEW! Technical Article No. 5

LC/MS Analysis of Various Low-Molecular Weight Cationic Compounds Using a Polymer-Based Carboxyl HILIC Column Shodex™ HILICpak™ VC-50 2D

Most low-molecular weight cationic compounds are hydrophilic and hardly retained under reversed phase mode. To compromise the problem, pre-column derivatization and ion-pair chromatography techniques are often used. VC-50 2D column provides a highly sensitive analysis of various low-molecular weight cationic compounds, while requiring simpler analytical conditions than the methods previously available.

Click here to read the full PDF version >>> Chromato News JAN 2019 – English

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SHODEX Chromato News December 2018

news | 01 December 2018
Tag: chromatonews shodex

Shodex Singapore Team wishing you and your family Happy Holidays

NEW! Technical Article No. 4

LC/MS Analysis of Oligonucleotides Using a Polymer-Based Diol Column
– Shodex™ HILICpak™ VN-50 2D

The oligonucleotide therapeutics have high expectations for the usage in not only treating genetic and metabolic disorders, but as for anti-cancer drugs and vaccines of various diseases. Shodex™ HILICpak™ VN-50 have been successfully measuring various oligosaccharides. And it is found to be also effective for the analysis of oligonucleotides.

Click here to read the full PDF version >>> Chromato News DEC 2018 – English

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SHODEX Chromato News October 2018

news | 01 October 2018
Tag: chromatonews shodex

New Application Data on Shodex.com Website!

FAST and ECONOMICAL Analysis

☆ LC/MS Analysis of Streptomycins (VC-50 2D)
★ LC/MS/MS Analysis of Citicoline (VT-50 2D)
☆ LC/MS Analysis of Low Molecular Weight Peptides (VC-50 2D)
Epoxy Resin (4) (Comparison of HK-404L and HK-404L + HK-401)
Biodegradable Polymer (4) (Polylactic Acid) (HK-404L)

Click here to read the full PDF version >>> Chromato News OCT 2018 – English

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SHODEX Chromato News September 2018

news | 01 September 2018
Tag: chromatonews shodex

NEW! Technical Article No. 3

LC/MS Analysis of Various Hydrophilic Compounds Using a Polymer-Based Amino Column
– Shodex™ HILICpak™ VG-50 2D

High polar compounds in pharmaceutical and food products are hardly retained under Reversed Phased mode, and to compromise the problem, pre-column derivatization or addition of ion-pair reagent to the eluent are often applied. Drawbacks are the complicated and time taking process of the derivatization and increase of background level caused by the ion-pair reagent residues on the column and the flow-lines.

However, Shodex™ HILICpack™ VG-50 2D, provides the solution with its advantageous analytical features when used under alkaline conditions. LC/MS with ammonia water/acetonitrile gradient elution is effective for good separation and high sensitivity analysis of various hydrophilic compounds. It can analyse saccharides, organic acids, and amino acid simultaneously, and able to monitor caffeine and water-soluble vitamins simultaneously. These can be achieved without using pre-column derivatization nor addition of ion-pairing reagent.

Click here to read the full PDF version >>> Chromato News SEP 2018 – English

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SHODEX Chromato News August 2018

news | 01 August 2018
Tag: chromatonews shodex

New Application Data on Shodex.com Website!

☆ Separation of Taurine and Inositol (NH2P-50 4E)
★ Comparison of Analysis of Glucosamine Hydrochloride between Polymer-based Amino Columns vs Silica-based Amino Columns
Fucoidan (SB-806M HQ)
★ Analysis of Cyclodextrins According to Japan’s Specifications and Standards for Food Additives Method (KS-802)
Galactooligosaccharides (2) (KS-801, NH2P-50 4E)
Xylooligosaccharides (3) (SH1011)
Xylooligosaccharides (2) (VN-50 4D)
★ Chitosan-Oligosaccharides (Chitooligosaccharides) (3) (VN-50 4D)
N-Acetylchitooligosaccharides (2) (VN-50 4D)

Click here to read the full PDF version >>> Chromato News AUG 2018 – English

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SHODEX Chromato News June 2018

news | 01 June 2018
Tag: chromatonews shodex

Introducing the New PROTEIN LW-403 4D!

Analyze proteins more rapidly and more economically with the newly optimized Shodex™ PROTEIN column: LW-403 4D.

DURABILITY Tested by Number of Sample Injections (LW-403 4D)
★ Calibration Curve for LW-403 4D (Proteins)
2X FASTER SEC Separation of IgG than Conventional Protein Column
2X FASTER SEC Separation of Standard Proteins than Conventional Protein Column

Click here to read the full PDF version >>> Chromato News JUN 2018 – English

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SHODEX Chromato News May 2018

news | 01 May 2018
Tag: chromatonews shodex

☪️RAMADAN MUBARAK – HAPPY RAMADAN ☪️
May the spirit of Ramadan show the way to peace and harmony.

4 New Application Data on Shodex.com Website!

☆ Assay of Polyethylene Glycol 3350 According to USP Method (SB-802.5 HQ)
★ Measurement of Apparent Weight-Average Molecular Weight and Polydispersity of Polyethylene Glycol 3350 According to USP Method (SB-803 HQ)
Colominic Acid (SB-804 HQ)
Hydroxypropyl-β-cyclodextrin (SB-802.5 HQ)
☆ Effects of Temperature on Retention Time (SI-35 2B)
★ Calibration Curves (SI-35 2B)

Click here to read the full PDF version >>> Chromato News MAY 2018 – English

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SHODEX Chromato News April 2018

news | 01 April 2018
Tag: chromatonews shodex

LET’S GET WORKS DONE FASTER 😉
In this month’s newsletter, we introduce 2 new polymer-based columns for faster analysis, helping you & your team save time in your work and achieving better productivity.

Firstly, we have our new SUGAR SP0810 8C column, which is an 8.0 x 100mm, USP L34 equivalent column that is suitable for USP Sorbitol as well as Fast carbohydrates analysis. It composes of strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead (Pb) form, and allows for the analysis of sucrose, glucose, galactose, fructose within 5 minutes due to the short 100mm length. If you are looking for a short USP L34 column, please feel free to contact us.

Next up, we have our IEC SP-FT 4A column, a cation exchange column for UHPLC modified with sulfopropyl groups on a non-porous polymethacrylate base material, for the ultra-rapid analysis of proteins and the like. Five standard proteins can be separated within 50 seconds under high flow rate condition of 3.0 mL / min.

Click here to read the full PDF version >>> Chromato News APR 2018 – English

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SHODEX Chromato News March 2018

news | 01 March 2018
Tag: chromatonews shodex

New Applications Data on Shodex.com Website!

☆ Analysis of Various Tween Type Surfactants (GF-210 HQ)
★LC/MS Analysis of Analysis of Tween Type Surfactants in IgG (ODP2 HP-2D)
☆ Standard Proteins (28) (Comparison of SP-FT 4A and SP-420N)
★ Effects of Flow Rate for Protein Analysis (SP-FT 4A)
☆ Ultra-rapid Analysis of Standard Proteins (SP-FT 4A)
★ Ultra-rapid Analysis of Hemoglobin A, F, S and C (SP-FT 4A)
☆ Analysis of Tagatose According to USP Method (SC1011)
★ Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)
☆ Polyamide (12) Nylon 6/9 (HK-HFIP404L)
★ Polyamide (13) Nylon 11 (HK-HFIP404L)
☆ Rapid Analysis of Anion Standards (SI-35 2B)
★ Analysis of Ionic Liquid (YS-50)
☆ LC/MS Analysis of Ionic Liquid (VC-50 2D)

Click here to read the full PDF version >>> Chromato News MAR 2018 – English

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SHODEX Chromato News February 2018

news | 01 February 2018
Tag: chromatonews shodex

Shodex Singapore Team wishes You
恭喜发财 🏮 Happy Lunar New Year! 🏮

Analysis method of Aggregates and Additives in Antibody Drugs

Antibody drugs are important potent therapeutic drugs. Because of their highly specific targeting capacity to the cancer cells, it can cause less side effects. However, it is known that they may aggregate to form dimers and other larger aggregates during manufacturing process and/or during storage period. Therefore, monitoring of such aggregates are important for quality control (QC) purposes. Shodex™ PROTEIN LW-803 demonstrated its superior separation capability for IgG monomers and its aggregates. While, Shodex™ ODP2 HP-2D proved its separation capability of analyzing non-ionic surfactant, Tween, in the presence of IgG and NaCl, without the necessity of deproteinization nor desalting sample pretreatment.

Click here to read the full PDF version >>> Chromato News FEB 2018- English

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SHODEX Chromato News January 2018

news | 01 January 2018
Tag: chromatonews shodex

Shodex Singapore Team wishes You a Prosperous and Joyful year ahead.
Happy New Year 2018!

Q: SHODEX makes Polymer-based HPLC columns, are they listed in Pharmacopeia-related Monographs? 【・_・?】

A: YES. The United States Pharmacopeia (USP), European Pharmacopeia (EP) and Japanese Pharmacopeia (JP) have established standard guidelines for analysis and are used widely by regulatory agencies and industries throughout the world to ensure products are of appropriate qualities. In this multi-page Special Edition, we will be bringing you a list of Shodex HPLC columns that may be suitable for analytical methods available in the above-mentioned Pharmacopeias, as well as examples of application data obtained with these specific types of columns. Examples include the analysis of Organic acids, Insulin Glargine, Mannitol, Dextrose and other sugars, Azithromycin, and more.

Click here to read the full PDF version >>> Chromato News JAN 2018- English

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SHODEX Chromato News November 2017

news | 01 November 2017
Tag: chromatonews shodex

6 New Application Data on Shodex.com Website!

★ Analysis of Choline and Acetylcholine (YS-50)
☆ LC/MS Analysis of Choline and Acetylcholine (VC-50 2D)
★ LC/MS/MS Analysis of Salacia Extract (1) (VC-50 2D)
☆ LC/MS/MS Analysis of Salacia Extract (2) (VC-50 2D)
★ LC/MS Analysis of Glucose and Gluconic Acid (VG-50 2D)
Biodegradable Polymer (3) (Polylactic Acid) (K-805L)
★ Comparison of Separation using HK-404L with

Click here to read the full PDF version >>> Chromato News NOV 2017 – English

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SHODEX – Lab Indonesia 2018

event | 25 October 2017
Venue:

Jakarta Convention Center, Jakarta, Indonesia

Date:

4th -6th April 2018

Booth No.:

G-01

Tag: Indonesia Pharmaceutical Shodex

Held alternately with Lab Asia, Lab Indonesia is one the leading laboratory exhibition in Southeast Asia which focuses highly on scientific instruments and laboratory equipment for various industries, including medical & pharmaceutical, F&B, oil & gas, bioscience and nano-science.
LabIndonesia – Indonesia Laboratory, Scientific Analytical Equipments and Services Exhibition and Conference is back for the 5thedition at Jakarta Convention Center, Jakarta, Indonesia. Jointly organized by ECMI ITE Asia Sdn Bhd and PT. ITE Exhibitions Indonesia, LabIndonesia aims to bring together scientific and analytical laboratory industry elites such as professional chemists, biochemists, microbiologists, analysts, research and development researchers, lab managers and quality control and assurance managers at a networking and resourceful platform.

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SHODEX – Thailand Lab International 2018

event | 25 October 2017
Venue:

BITEC, Bangkok International Trade & Exhibition Centre, Bangkok, Thailand

Date:

12th – 14th September 2018

Booth No.:

TBA

Tag: Pharmaceutical Shodex Thailand

Thailand LAB INTERNATIONAL is organized by VNU Exhibitions Asia Pacific Co., Ltd. a joint venture between Jaarbeurs from the Netherlands and TCC Exhibition and Convention Center from Thailand. VNU Exhibitions Asia Pacific develops, with industry partners and exhibition organizers from around the world, executes and internationalizes trade show and conferences from animal production and processing to technology, from agriculture to innovations and from pets to medical and laboratory sectors. VNU Exhibitions Asia Pacific successfully applies its concepts and know-how across industries and in all Asian countries. Through its international network and global reach, VNU Exhibitions Asia Pacific builds marketplaces in Asia – for the world.

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SHODEX – India Lab Expo 2018

event | 25 October 2017
Venue:

HITEX, Hyderabad, India

Date:

6th – 8th September 2018

Booth No.:

TBA

Tag: India Pharmaceutical Shodex

India Lab expo, India’s largest exhibition on laboratory, scientific, analytical and biotechnology sector attracts international as well as Indian manufacturers and distributors. Decision makers from sectors like hospitals, diagnostic labs, oil & petroleum, chemical, cosmetics and Government departments can be met at the tradeshow.

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SHODEX Chromato News October 2017

news | 01 October 2017
Tag: chromatonews shodex

6 New Application Data on Shodex.com Website!

★ Monitoring Papain Digestion of Humanized Monoclonal IgG (LW-803)
☆ Analysis of High Molecular Impurities in Insulin Aspart According to Pharmacopeia (KW-802.5)
★ Analysis of Preservatives According to Analytical Methods of Food Additives in Foods (1) (C18P 4E)
☆ Analysis of Preservatives According to Analytical Methods of Food Additives in Foods (2) (C18P 4E)
★ SEC Analysis of LMW Heparin (1) (Dalteparin Sodium) (KW-803 + KW-802.5)
☆ SEC Analysis of LMW Heparin (2) (Parnaparin Sodium) (KW-803 + KW-802.5)
★ Calibration Curves for GF-HQ Series (THF Eluent: PS)
☆ Calibration Curves for KD-800 Series (DMF Eluent: PMMA)
★ Calibration Curves for UT-800 Series (ODCB Eluent: PS)
☆ Calibration Curves for HT-800 Series (ODCB Eluent: PS)
★ Calibration Curve for AT-806MS (ODCB Eluent: PS)
☆ Calibration Curve for LF-404 (HFIP Eluent: PMMA)

Click here to read the full PDF version >>> Chromato News OCT 2017 – English

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SHODEX Chromato News September 2017

news | 01 September 2017
Tag: chromatonews shodex

Showa Denko to Launch New Shodex Analytical Columns

Showa Denko (SDK) will start selling next month, five new types of Shoedx high-performance liquid chromatography (HPLC) columns, namely, GPC HK-401, GPC HK-405 and GPC HK-HFIP404L packed columns for gel permeation chromatography (GPC: size exclusion chromatography [SEC] using organic solvents), as well as OHpak LB-805 packed column for gel filtration chromatography (GFC: SEC using aqueous solvents) and IEC SP-FT 4A packed column for ion exchange chromatography.
The five new analytical columns SDK developed this time feature more rapid analysis and higher sensitivity, which has been desired by analytical column users. SDK will continue meeting our customers’ needs by developing columns which enhances accuracy of analysis of target materials in a certain range of molecular weight, as well as those which enable analysis of hardly-soluble polymers and rapid analysis which is desired by users in the fields of medicine manufacturing and biochemistry, even with general analyzers.
More information about the new products and application data will be included in future editions of Chromato News.
Please look forward to it!

New Application Data on Shodex.com Website!

Saccharides and Amino Acids (3) (KS-801)
☆ Analysis of Dextrose According to USP (SC1011)
★ Analysis of Ascorbic Acid Injection According to USP (DM-614)
☆ Analysis of Trehalose According to USP Method (KS-801)
★ Analysis of Lipoproteins in Serum (KW-804)
Polystyrene Standards (23) (HK-404L)
Poly(Methyl Methacrylate) (3) (HK-404L)
Poly(Butyl Methacrylate) (2) (HK-404L)
Styrene Butyl Methacrylate Copolymer (3) (HK-404L)
☆ LC/MS/MS Analysis of Ribavirin (VC-50 2D)
★ LC/MS Analysis of Iodide (VT-50 2D)
☆ LC/UV/MS Analysis of Oligo DNA (VN-50 2D)

Click here to read the full PDF version >>> Chromato News SEP 2017 – English

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SHODEX – Thailand Lab International 2017

event | 31 July 2017
Venue:

BITEC, Bangkok International Trade & Exhibition Centre, Bangkok, Thailand

Date:

6th – 8th September 2017 (Wednesday – Friday)

Tag: Pharmaceutical Shodex Thailand
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SHODEX – Analytical Vietnam 2017

event | 15 January 2017
Venue:

International Centre for Exhibitions, Hanoi, Vietnam

Date:

29th – 31st March 2017 (Wednesday – Friday)

Tag: Pharmaceutical Shodex Vietnam
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